From the Payflow Pro Developer's Guide:
User authentication failed. Error is caused by one or more of the following:
1. Login information is incorrect. Verify that USER, VENDOR, PARTNER, and PASSWORD have been entered correctly. VENDOR is your merchant ID and USER is the same as VENDOR unless you created a Payflow Pro user. All fields are case sensitive.
2. Invalid Processor information entered. Contact merchant bank to verify.
3. "Allowed IP Address" security feature implemented. The transaction is coming from an unknown IP address. See PayPal Manager online help for details on how to use Manager to update the allowed IP addresses.
4. You are using a test (not active) account to submit a transaction to the live PayPal servers.